Estrogenic Activity of Phenolic Additives Determined By an In Vitro Yeast Bioassay
Environmental Health Perspectives v.109, n.2, Feb01
Danielle Miller,1 Brian B. Wheals,1 Nicola Beresford,2 and John P. Sumpter2
1Centre for Environmental Research; and 2Department of Biological Sciences, Brunel University, Uxbridge, Middlesex, United Kingdom
Abstract
We used a recombinant yeast estrogen assay to assess the activity of 73 phenolic additives that are used as sunscreens, preservatives, disinfectants, antioxidants, flavorings, or for perfumery. Thirty-two of these compounds displayed activity: 22 with potencies relative to 17ß-estradiol, ranging from 1/3,000 to < 1/3,000,000, and 10 compounds with an impaired response that could not be directly compared with 17ß-estradiol. Forty-one compounds were inactive. The major criteria for activity appear to be the presence of an unhindered phenolic OH group in a para position and a molecular weight of 140-250 Da. Key words: estrogenic activity, phenolic additives, recombinant yeast assay. Environ Health Perspect 109:133-138 (2001). [Online 19 January 2001]
http://ehpnet1.niehs.nih.gov/docs/2001/109p133-138miller/abstract.html
Address correspondence to J.P. Sumpter, Department of Biological Sciences, Brunel University, Uxbridge, Middlesex, UB8 3PH, UK. Telephone: 44 1 895 27 4000. Fax: 44 1 895 274348. E-mail: John.Sumpter@brunel.ac.ukThis work was supported in part under contract with the U.K. Department of Trade and Industry as part of the Government Chemist Programme.
Received 28 March 2000; accepted 11 September 2000.
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