Immunoprophylaxis in fish by injection
of mouse antibody genes
Nature Biotechnology Nov00 v.18, n.11
Niels Lorenzen1, Pauline M. Cupit2, Katja Einer-Jensen1, Ellen Lorenzen1, Peter Ahrens3, Christopher J. Secombes4 & Charles Cunningham2
1. Danish Veterinary Laboratory, Hangøvej 2, DK-8200 Aarhus N, Denmark.
2. Sars International Centre for Marine Molecular Biology, High Technology Centre, 5008 Bergen, Norway .
3. Danish Veterinary Laboratory, Bülowsvej 27, DK-1790 V, Copenhagen, Denmark.
4. Department of Zoology, University of Aberdeen, Aberdeen AB24 2TZ, Scotland, UK.
Correspondence should be addressed to N Lorenzen. e-mail: nl@svs.dk
Antibodies are a crucial part of the body's specific defense against infectious diseases and have considerable potential as therapeutic and prophylactic agents in humans and animals. The development of recombinant single-chain antibodies allows a genetic application strategy for prevention of infectious diseases. To test this in a fish model, a gene construct encoding a neutralizing single-chain antibody to the fish-pathogenic rhabdovirus VHSV (viral hemorrhagic septicemia virus) was administered to rainbow trout by intramuscular injection of plasmid DNA. Circulating recombinant antibodies could later be detected in the fish, and protective immunity to the viral disease was established.
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