Mindfully.org  

Home | Air | Energy | Farm | Food | Genetic Engineering | Health | Industry | Nuclear | Pesticides | Plastic
Political | Sustainability | Technology | Water
PCE contamination


Transgenic DNA introgressed into 
traditional maize landraces in Oaxaca, Mexico. 

Nature v.414, 541-543 29nov01

[Background articles on and by Ignacio Chapela]

Quist, David, Chapela, Ignacio. 

 


mindfully.org note: 
This paper was withdrawn by the editor of Nature on the advice of one out of three expert reviewers. This was NOT a case of fraud or an unworthy study. The withdrawal came as a direct result of industry pressure. It is the judgment of mindfully.org that this paper is highly significant and should serve as a warning to all those who place a high value on maintaining ancient and modern varieties of any type of plant. Please realize that the genetic engineering industry is conducting a test using the genetic stock that all humans and animals depend upon for sustenance. They are polluting everything without concern or knowledge of the consequences. In spite of the claims of safety and regulations, there is no precedence for them to know what questions to ask in order to know what the consequences are--and there will be consequences.

Greed has blinded them and caused them to think they can manipulate and duplicate nature, which of course, will never be possible. 

Suggested reading:
Science journal Nature accused over GM article JAMES MEEK / The Guardian (UK) 8jun02


The following is the note from the Nature editor

In our 29 November issue, we published the paper "Transgenic DNA introgressed into traditional maize landraces in Oaxaca, Mexico" by David Quist and Ignacio Chapela. Subsequently, we received several criticisms of the paper, to which we obtained responses from the authors and consulted referees over the exchanges. In the meantime, the authors agreed to obtain further data, on a timetable agreed with us, that might prove beyond reasonable doubt that transgenes have indeed become integrated into the maize genome. The authors have now obtained some additional data, but there is disagreement between them and a referee as to whether these results significantly bolster their argument.

In light of these discussions and the diverse advice received, Nature has concluded that the evidence available is not sufficient to justify the publication of the original paper. As the authors nevertheless wish to stand by the available evidence for their conclusions, we feel it best simply to make these circumstances clear, to publish the criticisms, the authors' response and new data, and to allow our readers to judge the science for themselves.

Editor, Nature


We obtained positive PCR amplification using primers specific for p-35S (35S promoter (p-35S) from the cauliflower mosaic virus) in five of the seven Mexican maize samples tested. Four criollo samples showed weak albeit clear PCR amplification, whereas the Diconsa sample yielded very strong amplification comparable in intensity to transgenic-positive Bt1 and RR1 controls. The historical negative control (data not shown) and the contemporary sample from Cuzco, Peru, were both invariably negative. Low PCR amplification from landraces was due to low transgenic abundance (that is, a low percentage of kernels in each cob), not to differential efficiency in the reaction, as demonstrated by internal control amplification of the maize-specific alpha zein protein 1 gene (zp1).(ref.2156)

It should also be noted that the author of the Nature article, Ignacio H. Chapela, is on the Board of Directors of the Pesticide Action Network North America (PANNA), an activist group.

November 29, 2001

Not Included in this file: PDF file, complete with figures here: Transgenic DNA introgressed into traditional maize landraces in Oaxaca, Mexico

*Department of Environmental Science, Policy and Management, University of California, Berkeley, California 94720-3110, USA

Concerns have been raised about the potential effects of transgenic introductions on the genetic diversity of crop landraces and wild relatives in areas of crop origin and diversification, as this diversity is considered essential for global food security. Direct effects on non-target species1, 2, and the possibility of unintentionally transferring traits of ecological relevance onto landraces and wild relatives have also been sources of concern3, 4. The degree of genetic connectivity between industrial crops and their progenitors in landraces and wild relatives is a principal determinant of the evolutionary history of crops and agroecosystems throughout the world5, 6. Recent introductions of transgenic DNA constructs into agricultural fields provide unique markers to measure such connectivity. For these reasons, the detection of transgenic DNA in crop landraces is of critical importance. Here we report the presence of introgressed transgenic DNA constructs in native maize landraces grown in remote mountains in Oaxaca, Mexico, part of the Mesoamerican centre of origin and diversification of this crop7-9.

In October and November 2000 we sampled whole cobs of native, or 'criollo', landraces of maize from four standing fields in two locations of the Sierra Norte de Oaxaca in Southern Mexico (samples A1–A3 and B1–B3), more than 20 km from the main mountain-crossing road that connects the cities of Oaxaca and Tuxtepec in the Municipality of Ixtlán. As each kernel results from ovule fertilization by individual pollen grains, each pooled criollo sample represents a composite of 150–400 pollination events. One additional bulk grain sample (K1) was obtained from the local stores of the Mexican governmental agency Diconsa (formerly the National Commission for Popular Subsistence), which distributes subsidized food throughout the country. Negative controls were cob samples of blue maize from the Cuzco Valley in Peru (P1) and a 20-seed sample from an historical collection obtained in the Sierra Norte de Oaxaca in 1971 (H1). Positive controls were bulk grain samples of Yieldgard Bacillus thuringiensis (Bt)-maize (Bt1; Monsanto Corporation) and Roundup-Ready maize (RR1; Monsanto Corporation) obtained from leftover stock for the 2000 planting season in the United States. Using a polymerase chain reaction (PCR)-based approach, we first tested for the presence of a common element in transgenic constructs currently on the market—the 35S promoter (p-35S) from the cauliflower mosaic virus (CMV). The high copy number and widespread use of p-35S in synthetic vectors used to incorporate transgenic DNA during plant transformation make it an ideal marker to detect transgenic constructs10-12.

We obtained positive PCR amplification using primers specific for p-35S in five of the seven Mexican maize samples tested. Four criollo samples showed weak albeit clear PCR amplification, whereas the Diconsa sample yielded very strong amplification comparable in intensity to transgenic-positive Bt1 and RR1 controls. The historical negative control (data not shown) and the contemporary sample from Cuzco, Peru, were both invariably negative. Low PCR amplification from landraces was due to low transgenic abundance (that is, a low percentage of kernels in each cob), not to differential efficiency in the reaction, as demonstrated by internal control amplification of the maize-specific alpha zein protein 1 gene (zp1). During the review period of this manuscript, the Mexican Government (National Institute of Ecology, INE, and National Commission of Biodiversity, Conabio) established an independent research effort. Their results, published through official government press releases, confirm the presence of transgenic DNA in landrace genomes in two Mexican states, including Oaxaca. Samples obtained by the Mexican research initiative from sites located near our collection areas in the Sierra Norte de Oaxaca also confirm the relatively low abundance of transgenic DNA in these remote areas. The governmental research effort analysed individual kernels, making it possible for them to quantify abundances in the range of 3–10%. Because we pooled all kernels in each cob, we cannot make such a quantitative statement, although low PCR amplification signal from criollo samples is compatible with abundances in this percentage range. Using a nested primer system, we were able to amplify the weak bands from all CMV-positive criollo samples sufficiently for nucleotide sequencing (GenBank accession numbers AF434747–AF434750), which always showed at least 98% homology with CMV p-35S constructs in commercially used vectors such as pMON273 (GenBank accession number X04879.1) and the K1 sample (accession number AF434746).

Further PCR testing of the same samples showed the presence of the nopaline synthase terminator sequence from Agrobacterium tumefasciens (T-NOS) in two of the six criollo samples (A3 and B2; GenBank accession numbers AF434752 and AF434751, respectively) and the Diconsa sample (K1; accession number AF434753). We detected the B. thuringiensis toxin gene cryIAb in one criollo sample (B3) (data not shown). We confirmed all of the PCR results through repeated testing.

We performed inverse PCR (iPCR) to reveal the various genomic contexts in which the CMV construct was embedded in the Oaxacan criollo maize. This method enabled us to sequence unknown DNA regions flanking the known p-35S sequence in each of the samples. For each sample, iPCR yielded 1–4 DNA fragments differing in size. We isolated these fragments from electrophoresis gels and attempted to sequence them individually, yielding sequences in eight cases (GenBank accession numbers AF434754–AF434761). Sequences adjacent to the CMV p-35S DNA were diverse, suggesting that the promoter was inserted into the criollo genome at multiple loci. When compared with GenBank (BLAST, February 2001), two sequences were similar to synthetic constructs containing regions of the adh1 gene found in transgenic maize currently on the market, such as Novartis Bt11 (samples A3 and K1). Notably, these two sequences had high homology with each other. Other sequences represented maize-native genomic DNA, including retrotransposon regions, whereas others showed no significant homology with any GenBank sequence. The diversity of transgenic DNA constructs present in criollo samples suggests the occurrence of multiple introgression events, probably mediated by pollination. In some of these events, the introgressed DNA appeared to have retained its integrity as an unaltered construct (as with adh1 (ref. 10), whereas in others the transgenic DNA construct seemed to have become re-assorted and introduced into different genomic backgrounds, possibly during transformation or recombination13. The apparent predominance of re-assorted sequences obtained in our study might be due to PCR bias for amplification of short fragments, as intact functional constructs are expected to be much longer.

Our results demonstrate that there is a high level of gene flow from industrially produced maize towards populations of progenitor landraces. As our samples originated from remote areas, it is to be expected that more accessible regions will be exposed to higher rates of introgression. Our discovery of a high frequency of transgene insertion into a diversity of genomic contexts indicates that introgression events are relatively common, and that the transgenic DNA constructs are probably maintained in the population from one generation to the next. The diversity of introgressed DNA in landraces is particularly striking given the existence in Mexico of a moratorium on the planting of transgenic maize since 1998. Whether the presence of these transgenes in 2000 is due to loose implementation of this moratorium, or to introgression before 1998 followed by the survival of transgenes in the population, remains to be established. The intentional release of large amounts of commercial transgenic seed into the environment since the mid-1990s represents a unique opportunity to trace the flow of genetic material over biogeographical regions, as well as a major influence on the future genetics of the global food system.

Further study of the impact of the gene flow from commercial hybrids to traditional landraces in the centres of origin and diversity of crop plants needs to be carefully considered with respect to the future of sustainable food production. Long-term studies should establish whether, or for how long, the integrity of the transgenic construct is retained, and whether the relatively low abundance of transgene introgression detected in the 2000 harvest cycle in Oaxaca will increase, decrease, or remain stable over time.

Methods

Extraction and purification of genomic DNA For each cob (sample), all kernels (152–384 kernels per cob) were ground to a fine powder using a steel miller to obtain a pooled sample. Three hundred seeds were also ground from each of the bulk samples, except for the historical negative control, which consisted of 20 seeds. Before use with each sample, millers were thoroughly washed, soaked in 10% sodium perchlorate for 30 min, rinsed and then autoclaved. Genomic DNA was extracted from 100 mg of the powder as described elsewhere10, with an added purification step using a Geneclean I Kit (Bio 101).

Polymerase chain reaction For protocol I, amplification reactions using 50–100 ng of extracted genomic DNA were carried out in 25 µl containing 1 PCR buffer (Promega), 2.5 mM MgCl2, 0.2 mM of each dNTP, 0.5 µM of each primer and 0.625 U of Platinum Taq Polymerase (GibcoBRL). We used a water negative control to verify that reactions were free of contamination. Amplifications were performed on a PTC-100 thermal cycler (MJ Research) with the following parameters: initial denaturation at 95 °C for 2 min; 40 cycles each with denaturing at 95 °C for 45 s, annealing for 1 min at 60 °C/56 °C for CMV/NOS, respectively, extension at 72 °C for 1 min; and a final extension for 5 min at 72 °C. For protocol II, where low amplicon yields were obtained, amplification was repeated as in protocol I but with only 20–25 cycles, followed by a re-amplification or nested amplification of a 1:250 dilution of the PCR products in a new reaction mix with partially or wholly nested primers for 10–15 cycles. Primers cm01 (5'-CACTACAAATGCCATCAT TGCGATA-3') and cm02 (5'-CTTATATAGAGGAAGGGTCTTGCGA-3')10 were used to detect CMV 35S promoters with protocol I. With protocol II we used nested primer pairs mp3 (5'-TCATCCCTTAC GTCAGTGGAGATAT-3') and mp4 (5'-GATAAAGGAAAGGCCATCGTTGAAG-3')12, and cm02 and mp4. Primers cm01 and cr02 (5'-CTCTCGGCGTAGATTTGGTACA-3')10 were used to detect the cryIAb synthetic gene. Primers zp01 (5'-TGCTTGCATTGTTCGC TCTCCTAG-3') and zp02 (5'-GTCGCAGT GACATTGTGGCAT-3')10 were used to amplify the maize-specific alpha zein protein 1 gene (zp1) as an external control for the presence of maize DNA and the efficiency of the reaction.

Inverse PCR Inverse PCR reactions were modified from previously described protocols14, 15. Genomic DNA for iPCR was digested with EcoRV (Promega), which targets a single digestion site internal to the p-35S. Restriction fragments were self-ligated with T4 DNA Ligase (Promega) (14 °C, 18 h), followed by heat inactivation (75 °C, 15 min) and phenol extraction. The purified, circularized products were resuspended in 10 µl 1:10 TE (10 mmol l-1 Tris, pH 8.5; 1 mmol l-1 EDTA). PCR amplification was performed using primers designed specifically for the CMV 35S promoter iCMV1 (5'-ACGTCTTCAAA GCAAGTGGA-3'), iCMV2 (5'-AGTGACAGATAGGATCGGGAAT-3') or iCMV3 (5'-GGAGAGGACACGCTGAAATC-3'), iCMV4 (5'-TAGTGGGATTGTGCGTCATC-3'). These primer pairs were designed to amplify outwards of the 35S promoter, downstream and upstream, respectively.

Nucleotide sequencing All nucleotide sequencing was carried out at the University of California at San Francisco Comprehensive Cancer Center, Genome Analysis Core Facility. All sequences mentioned above are available on the NCBI GenBank server ( http://www.ncbi.nlm.nih.gov ).

Supplementary information is available on Nature's World-Wide Web site ( http://www.nature.com ) or as paper copy from the London editorial office of Nature.

Received 26 July 2001;accepted 31 October 2001

References

1. Losey, J. E., Raynor, L. S. & Carter, M. E. Transgenic pollen harms monarch larvae. Nature 399, 214 (1999).

2. Saxena, D., Flores, S. & Stotzky, G. Insecticidal toxin in root exudates from Bt corn. Nature 402, 480 (1999).

3. Ellstrand, N. C. When transgenes wander, should we worry? Plant Physiol. 125, 1543-1545 (2001).

4. Doebley, J. Molecular evidence for gene flow among Zea species--genes transformed into maize through genetic engineering could be transferred to its wild relatives, the Teosintes. Bioscience 40, 443-448 (1990).

5. Ellstrand, N. C., Prentice, H. C. & Hancock, J. F. Gene flow and introgression from domesticated plants into their wild relatives. Annu. Rev. Ecol. Syst. 30, 539-563 (1999).

6. White, S. & Doebley, J. Of genes and genomes and the origin of maize. Trends Genet. 14, 327-332 (1998).

7. Wang, R.-L., Stec, A., Hey, J., Lukens, L. & Doebley, J. The limits of selection during maize domestication. Nature 398, 236-239 (1999).

8. Piperno, D. R. & Flannery, K. V. The earliest archaeological maize (Zea mays L.) from highland Mexico: new accelerator mass spectrometry dates and their implications. Proc. Natl Acad. Sci. USA 98, 2101-2103 (2001).

9. Iltis, H. From teosinte to maize: the catastrophic sexual transmutation. Science 222, 886-894 (1983).

10. Matsuoka, T. et al. A method of detecting recombinant DNAs from four lines of genetically modified maize. Shokuhin Eiseigaku Zasshi 41, 137-143 (2000).

11. Gachet, E., Martin, G. G., Vigeau, F. & Meyer, G. Detection of genetically modified organisms (GMOs) by PCR: a brief review of methodologies available. Trends Food Sci. Technol. 9, 380-388 (1999).

12. Anonymous Development of Methods to Identify Foods Produced by Means of Genetic Engineering EU Project SMT4-CT96-2072 (Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Berlin, 1999).

13. Pawlowski, W. P. & Somers, D. A. Transgenic DNA integrated into the oat genome is frequently interspersed by host DNA. Proc. Natl Acad. Sci. USA 95, 12106-12110 (1998).

14. Hartl, D. L. & Ochman, H. in Methods in Molecular Biology (ed. Harwood, A.) 293-301 (Humana, Totowa, New Jersey, 1996).

15. Zimmermann, A., Lüthy, L. & Pauli, U. Event specific transgene detection in Bt11 corn by quantitative PCR at the integration site. Lebensm.-Wiss. Technol. 33, 210-216 (2000).

Acknowledgements. We thank the Union de Comunidades Zapoteco Chinanteca (UZACHI) for access to their field laboratory, Y. Lara (Estudios Rurales y Asesoría, Oaxaca) for facilitation, A. King for Peruvian maize samples and CIMMYT maize germplasm bank for the historical control.

Competing interests statement. The authors declare that they have no competing financial interests.

Related articles: Transgenic corn found growing in Mexico

CIMMYT Response to Discovery of Transgenic Maize Growing In Mexico

 

source: http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v414/n6863/abs/414541a0_fs.html

If you have come to this page from an outside location click here to get back to mindfully.org